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QAZIPlasmid DNA Isolation Kit, MINIPREP 50Prep, P02-NIK01-50
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QAZIPlasmid DNA Isolation Kit, MINIPREP 50Prep, P02-NIK01-50
Quick AND Easy Isolation of plasmid DNA from recombinant E. coli cultures in less than 30 minutes
QAZIPlasmid DNA Isolation Kit, MINIPREP 50Prep, P02-NIK01-50
Item #: 73735219

QAZIPlasmid DNA Isolation Kit, MINIPREP 50Prep, P02-NIK01-50

95% of respondents would recommend this to a friend

Item #: 73735219

XCD 189

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Quick AND Easy Isolation of plasmid DNA from recombinant E. coli cultures in less than 30 minutes
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What Stands Out

High Yield
The QAZIPlasmid DNA Isolation Kit ensures exceptional yield, providing up to 50 preparations with superior plasmid recovery rates, ideal for researchers needing reliable results in genetic studies.
Easy Protocol
With a simplified step-by-step protocol, the kit minimizes time and effort, allowing users—both beginners and experienced professionals—to achieve consistent results quickly and efficiently.
Guaranteed Purity
The isolation kit offers top-notch plasmid purity with minimal contaminants, making it perfect for downstream applications like cloning and sequencing, ensuring high-quality DNA for critical experiments.

Product Details

Shop QAZIPlasmid DNA Isolation Kit, MINIPREP 50Prep, P02-NIK01-50 online at a best price in Grenada. B00S9ZISPW
  • Certified QAZIPlasmid DNA Isolation Kit, MINIPREP 50Prep, P02-NIK01-50
  • Enables quick and easy isolation of plasmid DNA from recombinant E. coli cultures
  • Can prepare up to 15 µg of high-quality plasmid DNA from 1 to 5 ml of E. coli culture in less than 30 minutes
  • Utilizes silica-membrane based technology in a spin column format or 96 well plate formats
  • Involves centrifugation, alkaline lysis, neutralization, and adsorption onto silica for DNA retention
  • Elution of bound DNA is achieved in water or Tris-EDTA buffer after a couple of wash steps
Country of OriginThis item will be imported from US
Date First AvailableMarch 17, 2022
What is in the boxQAZIPlasmid DNA Isolation Kit... For more details, please check description/product details

Who Should Buy?

Suitable For
  • Academic Researchers

    Ideal for researchers needing reliable plasmid isolation for academic projects, ensuring high-quality DNA yields.

  • Molecular Biologists

    Designed for molecular biologists focusing on cloning or gene manipulation, providing efficient and effective DNA purification.

  • Laboratory Technicians

    Perfect for technicians performing routine plasmid isolations, simplifying workflows with easy-to-follow protocols.

Not Suitable For
  • Industrial Applications

    Not suitable for large-scale industrial plasmid isolations which may require more robust methods.

Product Description

QAZIPlasmid DNA Isolation Kit, MINIPREP 50Prep, P02-NIK01-50

About This Item

Introducing the QAZIPlasmid DNA Isolation Kit - MINIPREP 50Prep (P02-NIK01-50) Looking for a quick and easy solution to isolate plasmid DNA from your recombinant E. coli cultures? Look no further than the QAZIPlasmid DNA Isolation Kit. This kit allows you to prepare high-quality plasmid DNA in less than 30 minutes, extracting up to 15 µg of DNA from just 1 to 5 ml of E.

coli culture. The QAZIPlasmid DNA Isolation Kit utilizes silica-membrane based technology in a convenient spin column or 96 well plate format. The isolation process involves three simple steps: bacterial harvesting, alkaline lysis, and neutralization using a chaotropic salt.

First, bacterial cells are harvested by centrifugation, ensuring optimal yield and purity of the DNA. Then, the alkaline lysis step breaks open the cells and releases the plasmid DNA. Neutralization with chaotropic salt stabilizes the DNA and prepares it for binding to the silica membrane.

The cleared lysate is then passed over the membrane, where the DNA binds to the silica due to adsorption in the presence of high salt conditions. After a couple of wash steps to remove impurities, the bound DNA is eluted in water or Tris-EDTA buffer, ready for downstream applications. The QAZIPlasmid DNA Isolation Kit is perfect for a variety of applications, including plasmid purification, DNA isolation, and plasmid DNA preparation. It comes with a comprehensive plasmid extraction protocol to guide you through each step, ensuring successful DNA isolation every time. Whether you are performing research, DNA sequencing, genetic engineering, or other molecular biology studies, the QAZIPlasmid DNA Isolation Kit provides a reliable and efficient method for obtaining high-quality plasmid DNA.

Don't settle for anything less than the best. Choose the QAZIPlasmid DNA Isolation Kit - MINIPREP 50Prep (P02-NIK01-50) and experience quick, easy, and reliable plasmid DNA extraction.

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Customer Questions & Answers

  • Question: What is the QAZIPlasmid DNA Isolation Kit used for?

    Answer: The QAZIPlasmid DNA Isolation Kit is designed for the efficient extraction of plasmid DNA from bacterial cultures. It utilizes a simple alkaline lysis method, allowing researchers to isolate high-quality plasmid DNA for a variety of applications. This can include cloning, sequencing, and transformation, making it a valuable tool in molecular biology laboratories and academic research settings.
  • Question: How many preparations does the QAZIPlasmid DNA Isolation Kit provide?

    Answer: This kit is specifically formulated to perform up to 50 plasmid preparations. This high yield makes it ideal for labs conducting multiple experiments or for projects that require large amounts of plasmid DNA. Each preparation is designed to yield sufficient DNA for downstream applications, ensuring that researchers can rely on consistent results.
  • Question: Is the QAZIPlasmid DNA Isolation Kit suitable for all types of bacteria?

    Answer: Yes, the QAZIPlasmid DNA Isolation Kit is versatile and can be used with a variety of bacterial strains, particularly those that are competent for transformation with plasmid DNA. It is an excellent choice for common laboratory strains such as E. coli. However, it is always advisable to verify the compatibility with your specific bacterial strain for optimal results.
  • Question: What are the main components included in the QAZIPlasmid DNA Isolation Kit?

    Answer: The QAZIPlasmid DNA Isolation Kit includes several key components essential for plasmid extraction: Lysis Buffer, Neutralization Buffer, Wash Buffer, and Elution Buffer. Each component works in conjunction to lyse the bacterial cells, neutralize the lysate, wash away impurities, and finally elute the purified plasmid DNA, ensuring a high-purity product ready for your experimental needs.
  • Question: What is the recommended protocol for using the QAZIPlasmid DNA Isolation Kit?

    Answer: The recommended protocol includes the steps of growing a bacterial culture, harvesting the cells, and then using the Lysis Buffer to break open the cells. Following neutralization and washing, the plasmid DNA is eluted using the provided Elution Buffer. Detailed instructions are typically included within the kit, which guides users through each step, ensuring efficient and reproducible results.
  • Question: What applications can be performed using the isolated plasmid DNA?

    Answer: Isolated plasmid DNA from the QAZIPlasmid DNA Isolation Kit can be utilized in various applications, such as molecular cloning, transformation into competent cells, and performing PCR. It is also vital for sequencing procedures and construction of recombinant DNA, making it an essential part of genetic engineering and research in biotechnology.
  • Question: How long does the plasmid isolation process take with the QAZIPlasmid DNA Isolation Kit?

    Answer: The entire plasmid isolation process generally takes about 30 to 60 minutes. This quick turnaround time is crucial for labs that need to conduct multiple experiments in a limited time frame. The straightforward steps involved mean that researchers can efficiently prepare their samples without significant downtime, which enhances productivity in research environments.
  • Question: Can the QAZIPlasmid DNA Isolation Kit be used for high-throughput applications?

    Answer: Yes, the QAZIPlasmid DNA Isolation Kit is suitable for high-throughput applications, particularly due to its capacity for up to 50 preparations. This makes it an excellent choice for labs that require handling multiple samples simultaneously. Furthermore, adopting this kit can streamline workflows in environments focused on efficiency and reproducibility in molecular biology.
  • Question: What is the shelf life of the QAZIPlasmid DNA Isolation Kit?

    Answer: The shelf life of the QAZIPlasmid DNA Isolation Kit is generally indicated on the packaging. Typically, if stored properly according to the provided instructions, the kit remains effective for several months. Always check the expiration date and manufacturer’s recommendations to ensure the reagents are functioning optimally for your plasmid isolation needs.
  • Question: Where can I buy the QAZIPlasmid DNA Isolation Kit in Grenada?

    Answer: You can purchase the QAZIPlasmid DNA Isolation Kit from Ubuy in Grenada. Ubuy is a trusted online platform that offers a wide range of laboratory supplies, ensuring you can swiftly obtain this kit along with other essential products for your research work.

RxBiosciences DNA & RNA Extraction Kits Editorial Review

QAZIPlasmid DNA Isolation Kit, MINIPREP 50Prep, P02-NIK01-50 is designed for efficient plasmid DNA isolation with a 50-prep capacity. This kit ensures high purity of plasmid DNA, making it suitable for various downstream applications. It originates from the US, ensuring quality and reliability in your molecular biology work. With its simple protocol, users can achieve rapid and effective isolation of plasmid DNA with minimal steps—a perfect choice for laboratories seeking to enhance their genetic research.

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Pros

  • High yield of plasmid DNA
  • Simple and quick protocol
  • Suitable for various applications
  • Efficient for molecular biology work
  • Quality assurance with US origin

Cons

  • Limited variety in kit options

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XCD 189

Order now and get it around Sunday, September 13

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Features & Benefits

  • Offers quick and easy isolation of plasmid DNA
  • Can prepare up to 15?g of high quality plasmid DNA in less than 30 minutes
  • Utilizes silica-membrane based technology in a spin column or 96 well plate format
  • Harvests bacteria by centrifugation, alkaline lysis, and neutralization
  • Retains DNA using a membrane through adsorption onto silica in the presence of high salts
  • Elutes DNA in water or Tris-EDTA buffer

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